The Definitive Guide to cytometers
The Definitive Guide to cytometers
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Details-enriched characterization of cell populations. Morphological info from visuals can include towards the richness of flow cytometry info. One example is, the determine displays an otherwise regular apoptosis assay using Annexin V and PI, introducing mobile imaging to characterize cells in each population to expose morphologically distinctive capabilities. These insights could not are already obtained from multiplex staining alone.
Irrespective of whether you’re a starter or a specialist, explore our means to progress your investigation and know-how in flow cytometry.
(B) RAW 264.7 macrophages are frequently annotated inaccurately with regard to particle depend due to their complexity and noncircular borders. The number of singlets obtainable for Investigation boosts as soon as the consumer trains and employs a far more correct design.
Find new insights into your experimental data by visualizing populations utilizing combined fluorescence and morphology information. Our application interface wizards help downsampling, concatenation (merged files) and dimensionality resources that are developed in the software program for an intuitive, streamlined info Examination experience.
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Moreover, it truly is illuminating to see what number of doublets slip by means of traditional singlet gates, and good to confirm the status of unforeseen double-constructive situations in my Assessment."
Optimizing gating strategy. Even sturdy manual singlet gating is mistake-vulnerable and continues to be a subjective decision place in Pretty much all flow cytometry assays. Imaging may be used to confirm and change gates to incorporate only solitary cells of interest.
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Early indications of this contamination had initially been dismissed as debris. Backgating to discover new populations. Backgating imaged cells over the Attune CytPix Flow Cytometer also allows you to use morphological attributes to find out exciting subpopulations that may not be obvious from flow cytometry information on your own.
Perhaps most of all, these gatherings consist of cells of Plainly unique phenotypes which may lead to incorrect conclusions regarding double good gatherings (especially in exceptional populations).
AI-driven automated image Examination Together with the Attune Cytometric Program interprets occasion capabilities into unique morphology parameters that may be coupled with conventional fluorescence and scatter parameters to reveal really reproducible population alterations and one of a kind subsets.
We Earlier shown the power of imaging CAR-T/Ramos cell interactions. Allow’s look at just the population of best fascination, the double good gatherings, To find out more. We can now use extended morphological parameters (right here, circularity and skewness of intensity) to further look at the characteristics of such populations and refine gating on these functions, escalating details robustness.
Developed-in dimensionality reduction tools greater resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to verify expected population resolution in lysed whole human blood. User can see functions from Each individual cluster for Visible affirmation of mobile phenotypes.
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Gating on these prolonged parameters allows you to speedily and properly recognize populations of interest and ensure gating system with little if any manual overview. The Attune software program offers access to point out-of-the-artwork device Understanding Examination resources inside of an expertly built consumer interface, rendering it exceptionally easy to use.
Since the mobile line had Formerly been analyzed about the Attune CytPix Flow Cytometer, the scientists went back again to the photographs and had been in the position to document the microbial an infection at least five days before.
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What gave the impression to be a homogeneous population is definitely check here more various—an insight that ought to be regarded as when interpreting outcomes.